Differentiating CD8αβ T Cells from TCR-Transduced iPSCs for Cancer Immunotherapy

Methods Mol Biol. 2019;2048:81-84. doi: 10.1007/978-1-4939-9728-2_9.

Abstract

The use of induced pluripotent stem cells (iPSCs) as a cell source for producing cytotoxic T lymphocytes (CTLs) is expected to have advantages in the antigen specificity, rejuvenation profile, and reproducible number of CTLs. We have developed the way to differentiate CD8αβ T cells from TCR-transduced iPSCs (TCR-iPSCs). These T cells express monoclonal expression of the transduced TCR. Generating CD8αβ CTLs from TCR-iPSC could contribute to safe and effective allogeneic regenerative T cell immunotherapies.

Keywords: T cell differentiation; TCR transduction; iPSCs.

MeSH terms

  • Animals
  • CD8 Antigens / immunology
  • CD8 Antigens / metabolism
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line
  • Cell Separation / instrumentation
  • Cell Separation / methods
  • Coculture Techniques / instrumentation
  • Coculture Techniques / methods
  • Culture Media / metabolism
  • Cytokines / metabolism
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods
  • Genetic Vectors / genetics
  • Humans
  • Immunotherapy, Adoptive / methods
  • Induced Pluripotent Stem Cells / physiology*
  • Lentivirus / genetics
  • Mesenchymal Stem Cells
  • Mice
  • Neoplasms / immunology
  • Neoplasms / therapy
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Cytotoxic / metabolism
  • T-Lymphocytes, Cytotoxic / transplantation*
  • Transduction, Genetic / instrumentation
  • Transduction, Genetic / methods*
  • Transplantation, Homologous / methods

Substances

  • CD8 Antigens
  • CD8alphabeta antigen
  • Culture Media
  • Cytokines
  • Receptors, Antigen, T-Cell
  • Recombinant Proteins