Extreme resistance to Potato virus Y in potato carrying the Ry sto gene is mediated by a TIR-NLR immune receptor

Plant Biotechnol J. 2020 Mar;18(3):655-667. doi: 10.1111/pbi.13230. Epub 2019 Sep 4.

Abstract

Potato virus Y (PVY) is a major potato (Solanum tuberosum L.) pathogen that causes severe annual crop losses worth billions of dollars worldwide. PVY is transmitted by aphids, and successful control of virus transmission requires the extensive use of environmentally damaging insecticides to reduce vector populations. Rysto , from the wild relative S. stoloniferum, confers extreme resistance (ER) to PVY and related viruses and is a valuable trait that is widely employed in potato resistance breeding programmes. Rysto was previously mapped to a region of potato chromosome XII, but the specific gene has not been identified to date. In this study, we isolated Rysto using resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences single-molecule real-time sequencing). Rysto was found to encode a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal TIR domain and was sufficient for PVY perception and ER in transgenic potato plants. Rysto -dependent extreme resistance was temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.

Keywords: PVY; Ry sto; RenSeq; TIR-NLR immune receptor; extreme resistance; potato.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids / virology
  • Breeding
  • Disease Resistance*
  • Genes, Plant*
  • NLR Proteins / immunology
  • Plant Diseases / immunology
  • Plant Diseases / virology*
  • Plants, Genetically Modified / virology
  • Potyvirus / pathogenicity*
  • Solanum tuberosum / immunology*
  • Solanum tuberosum / virology

Substances

  • NLR Proteins