T-cell phenotypes are associated with serum IgE levels in Amish and Hutterite children

J Allergy Clin Immunol. 2019 Nov;144(5):1391-1401.e10. doi: 10.1016/j.jaci.2019.07.034. Epub 2019 Aug 8.

Abstract

Objectives: Amish children raised on traditional farms have lower atopy and asthma risk than Hutterite children raised on modern farms. In our previous study we established that the Amish environment affects the innate immune response to decrease asthma and atopy risk. Here we investigated T-cell phenotypes in the same Amish and Hutterite children as in our earlier study to elucidate how this altered innate immunity affects adaptive T cells.

Methods: Blood was collected from 30 Amish and 30 Hutterite age- and sex-matched children; cells were cryopreserved until analysis. Flow cytometry was used to analyze cell subsets. Atopy was determined based on allergen-specific and total IgE levels.

Results: Children exposed to Amish farms had increased activated regulatory CD4+ T-cell phenotypes, whereas conventional CD4 T cells expressed lower levels of costimulation molecules and other activation markers. The increase in numbers of circulating activated regulatory CD4+ T cells was associated with an increase in inhibitory receptors on monocytes in Amish, but not Hutterite, children. Strikingly, the Amish children had a higher proportion of CD28null CD8 T cells than the Hutterite children (P < .0001, nonparametric t test), a difference that remained even after accounting for the effects of age and sex (conditional log regression exponential β = 1.08, P = .0053). The proportion of these cells correlated with high T-cell IFN-γ production (rs = 0.573, P = .005) and low serum IgE levels (rs = -0.417, P = .025). Furthermore, CD28null CD8 T-cell numbers were increased in Amish children, with high expression of the innate genes TNF and TNF-α-induced protein 3 (TNFAIP3) in peripheral blood leukocytes.

Conclusion: Amish children's blood leukocytes are not only altered in their innate immune status but also have distinct T-cell phenotypes that are often associated with increased antigen exposure.

Keywords: Asthma; CD4 T cells; CD8 T cells; T-cell activation; adaptive immunity; atopy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adaptive Immunity
  • Adolescent
  • Allergens / immunology
  • Amish*
  • Cells, Cultured
  • Child
  • Environmental Exposure / adverse effects
  • Ethnic Groups*
  • Female
  • Humans
  • Hypersensitivity, Immediate / immunology*
  • Immunoglobulin E / blood*
  • Immunophenotyping
  • Male
  • Phenotype
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes / immunology*
  • Tumor Necrosis Factor alpha-Induced Protein 3 / genetics
  • Tumor Necrosis Factor alpha-Induced Protein 3 / metabolism*

Substances

  • Allergens
  • Immunoglobulin E
  • TNFAIP3 protein, human
  • Tumor Necrosis Factor alpha-Induced Protein 3