A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

Stem Cell Res. 2019 Aug;39:101522. doi: 10.1016/j.scr.2019.101522. Epub 2019 Aug 1.

Abstract

Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apolipoproteins E / genetics
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cell Line
  • Embryoid Bodies / cytology*
  • Genotyping Techniques / methods
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Microsatellite Repeats / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sendai virus / genetics

Substances

  • Apolipoproteins E