Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

Chatrawee Duangjan; Panthakarn Rangsinth; Xiaojie Gu; Shaoxiong Zhang; Michael Wink; Tewin Tencomnao

doi:10.1016/j.phymed.2019.153061

Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

Phytomedicine. 2019 Nov:64:153061. doi: 10.1016/j.phymed.2019.153061. Epub 2019 Jul 31.

Authors

Chatrawee Duangjan¹, Panthakarn Rangsinth¹, Xiaojie Gu², Shaoxiong Zhang³, Michael Wink⁴, Tewin Tencomnao⁵

Affiliations

¹ Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany; Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
² Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany.
³ College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany.
⁴ Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany. Electronic address: wink@uni-heidelberg.de.
⁵ Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: tewin.t@chula.ac.th.

PMID: 31401497
DOI: 10.1016/j.phymed.2019.153061

Abstract

Background: Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported.

Purpose: We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model.

Methods: The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts.

Results: GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans.

Conclusion: This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress.

Keywords: Aging; Antioxidants; Caenorhabditis elegans; DAF-16/FoxO; Glochidion zeylanicum; SKN-1/Nrf2.

MeSH terms

Animals
Antioxidants / metabolism
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism*
DNA-Binding Proteins / metabolism*
Flavonoids / analysis
Forkhead Transcription Factors / metabolism*
Longevity / drug effects
Malpighiales / chemistry*
Oxidative Stress / drug effects
Phenols / analysis
Plant Extracts / pharmacology*
Plant Leaves / chemistry
Plants, Medicinal
Reactive Oxygen Species / metabolism
Signal Transduction / drug effects*
Transcription Factors / metabolism*

Substances

Antioxidants
Caenorhabditis elegans Proteins
DNA-Binding Proteins
Flavonoids
Forkhead Transcription Factors
Phenols
Plant Extracts
Reactive Oxygen Species
Transcription Factors
daf-16 protein, C elegans
skn-1 protein, C elegans