Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a devastating disease of eggplant (Solanum spp.) and causes substantial losses worldwide. Although some genes or biological processes involved in the interaction between eggplant and V. dahliae have been identified in some studies, the underlying molecular mechanism is not yet clear. Here, we monitored the transcriptomic profiles of the roots of resistant S. sisymbriifolium plants challenged with V. dahliae. Based on the measurements of physiological indexes (T-SOD, POD and SSs), three time points were selected and subsequently divided into two stages (S_12 h vs. S_0 h and S_48 h vs. S_12 h). KEGG enrichment analysis of the DEGs revealed several genes putatively involved in regulating plant-V. dahliae interactions, including mitogen-activated protein kinase (MAPK) genes (MEKK1 and MAP2K1), WRKY genes (WRKY22 and WRKY33) and cytochrome P450 (CYP) genes (CYP73A/C4H, CYP98A/C3'H and CYP84A/F5H). In addition, a subset of genes that play an important role in activating V. dahliae defence responses, including Ve genes as well as genes encoding PR proteins and TFs, were screened and are discussed. These results will help to identify key resistance genes and will contribute to a further understanding of molecular mechanisms of the S. sisymbriifolium resistance response to V. dahliae.
Keywords: Defence-response genes; Solanum sisymbriifolium; Transcriptome; Verticillium dahliae.
Conflict of interest statement
Conflict of interestThe authors declare no competing interests, including financial and non-financial interests.
Transcriptome profiling of Gossypium barbadense inoculated with Verticillium dahliae provides a resource for cotton improvement.BMC Genomics. 2013 Sep 22;14:637. doi: 10.1186/1471-2164-14-637. BMC Genomics. 2013. PMID: 24053558 Free PMC article.
Large-scale identification of Gossypium hirsutum genes associated with Verticillium dahliae by comparative transcriptomic and reverse genetics analysis.PLoS One. 2017 Aug 2;12(8):e0181609. doi: 10.1371/journal.pone.0181609. eCollection 2017. PLoS One. 2017. PMID: 28767675 Free PMC article.
Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae.BMC Genomics. 2017 Jan 6;18(1):42. doi: 10.1186/s12864-016-3386-7. BMC Genomics. 2017. PMID: 28061745 Free PMC article.
Interactions between Verticillium dahliae and its host: vegetative growth, pathogenicity, plant immunity.Appl Microbiol Biotechnol. 2014 Aug;98(16):6921-32. doi: 10.1007/s00253-014-5863-8. Epub 2014 Jun 15. Appl Microbiol Biotechnol. 2014. PMID: 24928658 Review.
Physiological and molecular mechanism of defense in cotton against Verticillium dahliae.Plant Physiol Biochem. 2018 Apr;125:193-204. doi: 10.1016/j.plaphy.2018.02.011. Epub 2018 Feb 13. Plant Physiol Biochem. 2018. PMID: 29462745 Review.