Health Benefits of Lactobacillus gasseri CP2305 Tablets in Young Adults Exposed to Chronic Stress: A Randomized, Double-Blind, Placebo-Controlled Study

Nutrients. 2019 Aug 10;11(8):1859. doi: 10.3390/nu11081859.


Short-term administration of Lactobacillus gasseri CP2305 improves stress-associated symptoms and clinical symptoms in healthy young adults and in patients with irritable bowel syndrome, respectively. We evaluated the efficacy and health benefits of the long-term use of a tablet containing heat-inactivated, washed Lactobacillus gasseri CP2305 (CP2305) in healthy young adults. Sixty Japanese medical students (41 men and 19 women) preparing for the national examination for medical practitioners ingested CP2305-containing or placebo tablets once daily for 24 weeks. Intake of the CP2305 tablet significantly reduced anxiety and sleep disturbance relative to placebo, as quantitated by the Spielberger State-Trait Anxiety Inventory and the Pittsburgh Sleep Quality Index. Single-channel sleep electroencephalograms show that CP2305 significantly shortened sleep latency and wake time after sleep onset and increased the delta power ratio in the first sleep cycle. CP2305 also significantly lowered salivary chromogranin A levels compared with placebo. Furthermore, 16S rRNA gene sequencing of participant feces demonstrated that CP2305 administration attenuated the stress-induced decline of Bifidobacterium spp. and the stress-induced elevation of Streptococcus spp. We conclude that the long-term use of CP2305-containing tablets may improve the mental state, sleep quality, and gut microbiota of healthy adults under stressful conditions.

Keywords: fecal microbiota; healthy young adults; heat-inactivated Lactobacillus gasseri CP2305; mental health; psychobiotics; sleep quality; stress.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Bifidobacterium / metabolism
  • Chromogranin A / metabolism
  • Chronic Disease
  • Double-Blind Method
  • Female
  • Gastrointestinal Microbiome
  • Healthy Volunteers
  • Humans
  • Lactobacillus gasseri*
  • Male
  • Probiotics / administration & dosage*
  • RNA, Ribosomal, 16S / analysis
  • Saliva / chemistry
  • Sleep Wake Disorders / microbiology
  • Sleep Wake Disorders / psychology
  • Sleep Wake Disorders / therapy
  • Streptococcus / metabolism
  • Stress, Psychological / microbiology*
  • Stress, Psychological / psychology
  • Stress, Psychological / therapy*
  • Students, Medical / psychology
  • Tablets
  • Treatment Outcome
  • Young Adult


  • Chromogranin A
  • RNA, Ribosomal, 16S
  • Tablets

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