Assessment of MMP29 Gene Expression and Silver Nanoparticles Effects on Colon Cancer Cell Line (HT29)

J Gastrointest Cancer. 2020 Jun;51(2):560-563. doi: 10.1007/s12029-019-00289-8.


Background: Colon cancer is a major cause of death around the world. The evaluation of novel approaches on corresponding genes would be a vital strategy toward eradication of cancer cells. In this study, the toxicity of silver nanoparticle on the colon cancer cell line (HT29) and expression of matrix metalloproteinase (MMP29) gene was investigated.

Materials and methods: The silver nanoparticle (AgNPs) was synthesized and assessed by transmission electron microscopy (TEM). The cytotoxicity of synthesized AgNP on the HT29 cell line was evaluated using the MTT assay. Furthermore, the expression of MMP29 gene was investigated by the quantitative real-time PCR (RT-qPCR).

Results: The TEM results revealed that the fabricated AgNPs were mostly spherical in shape and had an average diameter of 22 nm. The results outlined that AgNPs significantly decreased the viability of cells in a dose- and time-dependent manner (p < 0.001). Additionally, we observed a significant difference among various concentrations.

Conclusion: The findings indicated that the green fabricated AgNPs have the potential as a promising approach toward the colon cancer therapy. Furthered studies are essential to evaluate against other cancer cell lines and genes participating in the cancer progress.

Keywords: AgNPs; Colon Cancer; Cytotoxicity; HT29; Matrix metalloproteinase 9.

MeSH terms

  • Cell Line, Tumor
  • Colonic Neoplasms / drug therapy*
  • Colonic Neoplasms / enzymology
  • Colonic Neoplasms / genetics
  • Gene Expression
  • HT29 Cells
  • Humans
  • Matrix Metalloproteinases / biosynthesis
  • Matrix Metalloproteinases / genetics
  • Metal Nanoparticles / therapeutic use*
  • Silver / administration & dosage


  • Silver
  • Matrix Metalloproteinases