miR-497-5p inhibits gastric cancer cell proliferation and growth through targeting PDK3

Biosci Rep. 2019 Sep 6;39(9):BSR20190654. doi: 10.1042/BSR20190654. Print 2019 Sep 30.

Abstract

MicroRNA plays an important role in gastric cancer (GC) development, while the function of miR-497-5p in this disease remains unknown. In the present study, we demonstrated miR-497-5p as a tumor suppressive microRNA in GC. miR-497-5p was down-regulated in GC tissues and its expression was associated with the disease stage. Inhibition of miR-497-5p promoted GC cell proliferation and growth. By contrast, miR-497-5p ectopic expression suppressed the proliferation and growth of GC cells. In addition, miR-497-5p inhibited DNA synthesis and enhanced apoptosis in GC cells. The cell cycle progression was suppressed by miR-497-5p. Mechanistically, miR-497-5p directly targeted and suppressed the expression of pyruvate dehydrogenase kinase 3 (PDK3), which is highly expressed in GC tissues. Over-expression of PDK3 promoted the proliferation of GC cells. Our study revealed that miR-497-5p inhibited GC cell proliferation and growth via targeting PDK3.

Keywords: DNA synthesis; PDK3; apoptosis; cell cycle; gastric cancer; miR-497-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Apoptosis / genetics
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Humans
  • MicroRNAs / genetics*
  • Middle Aged
  • Pyruvate Dehydrogenase Acetyl-Transferring Kinase / genetics*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Signal Transduction
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / pathology*

Substances

  • MIRN497 microRNA, human
  • MicroRNAs
  • PDK3 protein, human
  • Pyruvate Dehydrogenase Acetyl-Transferring Kinase
  • RNA, Small Interfering