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. 1988 Sep;43(3):184-8.
doi: 10.1007/BF02571318.

The Quantification of Gammacarboxyglutamic Acid Residues in Plasma-Osteocalcin

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The Quantification of Gammacarboxyglutamic Acid Residues in Plasma-Osteocalcin

B A Soute et al. Calcif Tissue Int. .

Abstract

In this paper we describe an assay procedure for determining the amount of gammacarboxyglutamic acid (Gla) residues in serum- or plasma-osteocalcin. The test includes removing by ethanol precipitation the majority of the proteins from the plasma (e.g., the Gla-containing coagulation factors) and the specific extraction of osteocalcin with the aid of immobilized immunopurified antibodies. It is demonstrated that the Gla-content of circulating osteocalcin from normal cows is similar to that of osteocalcin obtained from bone. Hence, the fact that part of the newly synthesized osteocalcin does not bind to the hydroxyapatite matrix in bone cannot be explained by an undercarboxylation of the molecule.

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References

    1. Methods Enzymol. 1984;107:548-51 - PubMed
    1. J Biol Chem. 1974 Jan 25;249(2):594-605 - PubMed
    1. Anal Biochem. 1982 Feb;120(1):1-7 - PubMed
    1. Int Arch Allergy Appl Immunol. 1966;29(2):185-9 - PubMed
    1. J Biol Chem. 1978 Dec 25;253(24):9063-8 - PubMed

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