Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots

Glycobiology. 2019 Nov 20;29(12):817-821. doi: 10.1093/glycob/cwz061.


Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.

Keywords: IgG glycome; biomarker; dried blood spots; high-throughput; ultra-performance liquid chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Dried Blood Spot Testing*
  • Glycosylation
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / chemistry*
  • Immunoglobulin G / isolation & purification


  • Immunoglobulin G