Injectable Amniotic Membrane/Umbilical Cord Particulate for Knee Osteoarthritis: A Prospective, Single-Center Pilot Study

Pain Med. 2019 Nov 1;20(11):2283-2291. doi: 10.1093/pm/pnz143.

Abstract

Objective: To evaluate the short-term safety and effectiveness of amniotic membrane/umbilical cord particulate (AMUC) in managing pain in patients with various severities of knee osteoarthritis (OA).

Design: Single-center, prospective, investigator-initiated pilot study.

Setting: Private practice.

Subjects: A total of 20 knee OA patients aged ≥18 years were enrolled with pain >40 mm, as determined by the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC)-A.

Methods: Patients received an ultrasound-guided, intra-articular injection of 50 mg of AMUC particulate reconstituted in 2 mL of preservative-free saline. All patients were then monitored at six weeks, 12 weeks, and 24 weeks postinjection. Patients who did not show >30% reduction in pain received a second injection of AMUC at six weeks. WOMAC, Patient Global Assessment, medication usage, and magnetic resonance imaging (MRI) were assessed.

Results: Knee OA pain significantly decreased from 74.3 ± 17.2 at baseline to 45.0 ± 25.4 at six weeks (P < 0.01), 35.4 ± 26.6 at 12 weeks (P < 0.001), and 37.4 ± 26.7 at 24 weeks (P < 0.001). This pain reduction was associated with a significant improvement in physical function (WOMAC-C) at all time points (P < 0.05) and stiffness (WOMAC-B) at 12 weeks (P = 0.01). Eleven patients received a second injection, which was significantly correlated with body mass index >30 kg/m2 (P = 0.025). MRI evaluation of the overall population revealed an improvement in the severity of bone marrow lesions in seven patients. No adverse events were observed.

Conclusions: AMUC particulate injection relieved pain and improved physical function in patients with symptomatic knee OA.

Keywords: Amniotic Membrane; Knee Joint Pain; Orthopedics; Osteoarthritis; Treatment Outcome; Umbilical Cord; WOMAC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Humans
  • Injections, Intra-Articular / methods
  • Knee Joint / drug effects*
  • Male
  • Middle Aged
  • Osteoarthritis, Knee / drug therapy*
  • Pain / drug therapy*
  • Pain Management / methods
  • Pain Measurement / methods
  • Pilot Projects
  • Treatment Outcome