Interaction of High Molecular Weight Chondroitin Sulfate From Human Aorta With Plasma Low Density Lipoproteins

Atherosclerosis. 1988 Oct;73(2-3):113-24. doi: 10.1016/0021-9150(88)90032-9.

Abstract

Aortic glycosaminoglycans were separated into fractions of increasing molecular weights containing heparan sulfate or chondroitin 4/6-sulfate + dermatan sulfate. When these fractions were added to plasma low density lipoproteins (LDL) in the presence of Ca2+, only chondroitin 4/6 sulfate + dermatan sulfate of high relative molecular weight produced turbidity. Treatment with testicular hyaluronidase abolished totally the formation of insoluble complex. When these glycosaminoglycans were applied to LDL-affinity columns in the presence of Ca2+, higher NaCl concentrations were necessary for the elution of the high relative molecular weight chondroitin sulfate. Heparan sulfate fractions did not produce turbidity when added to LDL solutions and were eluted from LDL-affinity columns at low NaCl concentrations. All these results suggest that besides the structure (or charge density), the molecular weight of the chondroitin sulfate chains is a relevant factor for the binding of this compound to LDL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aorta / metabolism*
  • Chemical Phenomena
  • Chemistry
  • Chondroitin / analogs & derivatives*
  • Chondroitin Sulfates / metabolism*
  • Chromatography, Agarose
  • Chromatography, DEAE-Cellulose
  • Dermatan Sulfate / analysis
  • Electrophoresis, Agar Gel
  • Glycosaminoglycans / analysis
  • Glycosaminoglycans / metabolism
  • Heparitin Sulfate / analysis
  • Humans
  • In Vitro Techniques
  • Lipoproteins, LDL / metabolism*
  • Molecular Weight

Substances

  • Glycosaminoglycans
  • Lipoproteins, LDL
  • Dermatan Sulfate
  • Chondroitin
  • Chondroitin Sulfates
  • Heparitin Sulfate