Exopolysaccharide Produced by Lactobacillus paracasei IJH-SONE68 Prevents and Improves the Picryl Chloride-Induced Contact Dermatitis

Molecules. 2019 Aug 16;24(16):2970. doi: 10.3390/molecules24162970.

Abstract

Allergic disease is one of the most important and common health problems worldwide. We have previously demonstrated that a fig leaf-derived lactic acid bacterium Lactobacillus (Lb.) paracasei IJH-SONE68 produces a novel exopolysaccharide (EPS). Furthermore, we have shown that the EPS inhibits the catalytic activity of hyaluronidase (EC 3.2.1.36) promoting inflammatory reactions. To evaluate the anti-allergy and anti-inflammatory effects of the EPS, in the present study, we employed the picryl-chloride-induced delayed-type (type IV) allergy model mice, which is used to evaluate the contact dermatitis. Oral administration of the EPS was observed to reduce the ear swelling in the model mice. We also observed that the overexpression of ear interleukin-4 (T helper (Th) 2 cytokine) mRNA and the increase in serum immunoglobulin E (IgE) are repressed. However, the expression of interferon-γ (Th1 cytokine) was not accelerated in all of the allergen-challenged model mice. The improvement may be responsible for the Th2 downregulation rather than the Th1 upregulation. In addition, the symptom of immediate-type (type I) allergy model mice was improved by oral administration of the IJH-SONE68 cell (data not shown). We can conclude that the IJH-SONE68-derived EPS is useful to improve the type I and IV allergies including atopic dermatitis.

Keywords: Lactobacillus paracasei; contact dermatitis; exopolysaccharide; lactic acid bacteria.

MeSH terms

  • Administration, Oral
  • Allergens / immunology
  • Allergens / metabolism
  • Animals
  • Anti-Allergic Agents / isolation & purification
  • Anti-Allergic Agents / pharmacology*
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Dermatitis, Contact / etiology
  • Dermatitis, Contact / immunology
  • Dermatitis, Contact / pathology
  • Dermatitis, Contact / prevention & control*
  • Dermatologic Agents / isolation & purification
  • Dermatologic Agents / pharmacology*
  • Disease Models, Animal
  • Ear
  • Gene Expression / drug effects
  • Hyaluronoglucosaminidase / antagonists & inhibitors
  • Hyaluronoglucosaminidase / immunology
  • Hyaluronoglucosaminidase / metabolism
  • Immunoglobulin E / blood
  • Immunoglobulin E / genetics
  • Interferon-gamma / genetics
  • Interferon-gamma / immunology
  • Interleukin-4 / genetics
  • Interleukin-4 / immunology
  • Lacticaseibacillus paracasei / chemistry*
  • Lacticaseibacillus paracasei / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Picryl Chloride / administration & dosage
  • Polysaccharides, Bacterial / isolation & purification
  • Polysaccharides, Bacterial / pharmacology*
  • Th1 Cells / drug effects
  • Th1 Cells / immunology
  • Th2 Cells / drug effects
  • Th2 Cells / immunology

Substances

  • Allergens
  • Anti-Allergic Agents
  • Anti-Inflammatory Agents
  • Dermatologic Agents
  • Il4 protein, mouse
  • Polysaccharides, Bacterial
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma
  • Hyaluronoglucosaminidase
  • Picryl Chloride