Oblique-plane single-molecule localization microscopy for tissues and small intact animals

Nat Methods. 2019 Sep;16(9):853-857. doi: 10.1038/s41592-019-0510-z. Epub 2019 Aug 19.


Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • A549 Cells
  • Animals
  • Brain / diagnostic imaging*
  • Caenorhabditis elegans / metabolism*
  • Drosophila melanogaster / metabolism*
  • Female
  • Fishes / metabolism*
  • Humans
  • Imaging, Three-Dimensional
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence / methods*
  • Retina / diagnostic imaging*
  • Single Molecule Imaging / methods*