Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

Commun Biol. 2019 Aug 9;2:305. doi: 10.1038/s42003-019-0538-8. eCollection 2019.


Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.

Keywords: Biotechnology; ESCRT; Fungal biology; Proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology
  • Caspofungin / pharmacology
  • Cell Survival / drug effects
  • Cell Wall / drug effects
  • Cell Wall / metabolism*
  • Endosomal Sorting Complexes Required for Transport / metabolism
  • Extracellular Vesicles / drug effects
  • Extracellular Vesicles / metabolism*
  • Mutation / genetics
  • Proteomics
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism*
  • Stress, Physiological / drug effects


  • Antifungal Agents
  • Endosomal Sorting Complexes Required for Transport
  • Caspofungin