Hydrogel-Based Therapy for Brain Repair After Intracerebral Hemorrhage

Transl Stroke Res. 2020 Jun;11(3):412-417. doi: 10.1007/s12975-019-00721-y. Epub 2019 Aug 20.


We assessed an injectable gelatin hydrogel containing epidermal growth factor (Gtn-EGF) as a therapy for intracerebral hemorrhage (ICH). ICH was induced in rats via collagenase injection into the striatum. Two weeks later, Gtn-EGF was injected into the cavitary lesion. The hydrogel filled ICH cavities without deforming brain tissue. Immunostaining demonstrated that neural precursor cells could migrate into the matrix, and some of these differentiated into neurons along with the appearance of astrocytes, oligodendrocytes, and endothelial cells. Sensorimotor tests suggested that Gtn-EGF improved neurological recovery. This study provides proof-of-principle that injectable biomaterials may be a translationally relevant approach for treating ICH.

Keywords: Injectable hydrogel; Neural precursors; Stroke cavity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brain / drug effects*
  • Brain / pathology*
  • Cerebral Hemorrhage / drug therapy*
  • Cerebral Hemorrhage / pathology*
  • Disease Models, Animal
  • Drug Delivery Systems / instrumentation*
  • Drug Delivery Systems / methods*
  • Epidermal Growth Factor / administration & dosage*
  • Gelatin / administration & dosage
  • Hydrogels / administration & dosage
  • Male
  • Neuroprotective Agents / administration & dosage*
  • Rats, Sprague-Dawley


  • Hydrogels
  • Neuroprotective Agents
  • Epidermal Growth Factor
  • Gelatin