Functional Analyses of Embryonic Cerebrospinal Fluid Proteins

Methods Mol Biol. 2019:2044:51-60. doi: 10.1007/978-1-4939-9706-0_3.

Abstract

The embryonic cerebrospinal fluid (eCSF) influences neuroepithelial cell behavior, affecting proliferation, differentiation, and survival. One major question to resolve in the field is to precisely describe the eCSF molecules responsible and to understand how these molecules interact in order to exert their functions. Here we describe an in vitro protocol to analyze the influence of eCSF components on neuroepithelium development.

Keywords: Chick embryo; Development; Embryonic cerebrospinal fluid; Mesencephalic explant; Neurogenesis; Protein interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Cerebrospinal Fluid Proteins / isolation & purification
  • Cerebrospinal Fluid Proteins / metabolism*
  • Cerebrospinal Fluid Proteins / physiology
  • Chick Embryo
  • Immunohistochemistry / methods
  • Neuroepithelial Cells / cytology*
  • Neurogenesis
  • Organ Culture Techniques / methods
  • Tegmentum Mesencephali / cytology
  • Tegmentum Mesencephali / embryology

Substances

  • Cerebrospinal Fluid Proteins