Array-Based Profiling of Proteins and Autoantibody Repertoires in CSF

Methods Mol Biol. 2019;2044:303-318. doi: 10.1007/978-1-4939-9706-0_19.

Abstract

Protein profiling enabled through affinity proteomics represents a powerful strategy for analysis of complex samples such as human body fluids. Cerebrospinal fluid (CSF) is the proximal fluid of the central nervous system and is commonly analyzed in the context of neurological diseases. Through the presence of brain-derived proteins, this fluid can offer insight into the physiological state of the brain. Here, we describe multiplex and flexible protein and autoantibody profiling approaches using suspension bead arrays. Through minimal sample processing, these methods enable high-throughput analysis of hundreds of samples and proteins in one single assay and thereby provide powerful approaches for discovery of disease-associated proteins and autoantigens.

Keywords: Affinity proteomics; Antibody arrays; Antigen arrays; Autoantibodies; Autoantibody profiling; Cerebrospinal fluid; Protein arrays; Protein profiling.

MeSH terms

  • Autoantibodies / cerebrospinal fluid*
  • Autoantibodies / immunology
  • Autoantigens / immunology*
  • Brain / metabolism
  • Humans
  • Protein Array Analysis / methods*
  • Proteome / analysis
  • Proteome / metabolism
  • Proteomics / methods*
  • Workflow

Substances

  • Autoantibodies
  • Autoantigens
  • Proteome