Inonotus obliquus attenuates histamine-induced microvascular inflammation

PLoS One. 2019 Aug 22;14(8):e0220776. doi: 10.1371/journal.pone.0220776. eCollection 2019.


Cell-to-cell communication is a key element of microvascular blood flow control, including rapidly carrying signals through the vascular endothelium in response to local stimuli. This cell-to-cell communication is negatively impacted during inflammation through the disruption of junctional integrity. Such disruption is associated with promoting the onset of cardiovascular diseases as a result of altered microvascular blood flow regulation. Therefore, understanding the mechanisms how inflammation drives microvascular dysfunction and compounds that mitigate such inflammation and dysfunction are of great interest for development. As such we aimed to investigate extracts of mushrooms as potential novel compounds. Using intravital microscopy, the medicinal mushroom, Inonotus obliquus was observed, to attenuate histamine-induced inflammation conducted vasodilation in second-order arterioles in the gluteus maximus muscle of C57BL/6 mice. Mast cell activation by C48/80 similarly disrupted endothelial junctions and conducted vasodilation but only histamine was blocked by the histamine antagonist, pyrilamine not C48/80 suggesting the importance of mast cell activation. Data presented here supports that histamine induced inflammation is a major disruptor of junctional integrity, and highlights the important anti-inflammatory properties of Inonotus obliquus focusing future assessment of mast cells as putative target for Inonotus obliquus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / isolation & purification
  • Agaricales / metabolism
  • Animals
  • Arterioles / drug effects
  • Basidiomycota / isolation & purification*
  • Basidiomycota / metabolism
  • Endothelium, Vascular / drug effects
  • Histamine / metabolism
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Intercellular Junctions / drug effects
  • Intercellular Junctions / metabolism
  • Male
  • Mast Cells / drug effects
  • Mast Cells / physiology
  • Mice
  • Mice, Inbred C57BL
  • Microvessels / drug effects*
  • Microvessels / immunology*
  • Pyrilamine / pharmacology
  • Vasodilation / drug effects


  • Histamine
  • Pyrilamine

Grants and funding

This work was funded by UNBC internal grants to GWP, CHL and SJ. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.