Viruses are essentially composed of a nucleic acid (segmented or not, DNA, or RNA) and a protein coat. Despite their simplicity, these small pathogens are responsible for significant economic and humanitarian losses that have had dramatic consequences in the course of human history. Since their discovery, scientists have developed different strategies to efficiently detect viruses, using all possible viral features. Viruses shape, proteins, and nucleic acid are used in viral detection. In this review, the development of these techniques, especially for plant and mammalian viruses, their strengths and weaknesses as well as the latest cutting-edge technologies that may be playing important roles in the years to come are described.
Keywords: biosensors; clustered regularly interspaced short palindromic repeats (CRISPR); enzyme-linked immunosorbent assay (ELISA); high-throughput sequencing (HTS); microscope; quantitative polymerase chain reaction (qPCR).
© 2019 WILEY Periodicals, Inc.