Preparation of isoquercitrin by biotransformation of rutin using α-L-rhamnosidase from Aspergillus niger JMU-TS528 and HSCCC purification

Prep Biochem Biotechnol. 2020;50(1):1-9. doi: 10.1080/10826068.2019.1655763. Epub 2019 Aug 23.


Isoquercitrin is a flavonoid with important applications in the pharmaceutical and nutraceutical industries. However, a low yield and high production cost hinders the industrial preparation of isoquercitrin. In the present study, isoquercitrin was prepared by biotransformation of rutin using α-L-rhamnosidase from Aspergillus niger JMU-TS528 combined with high-speed countercurrent chromatography (HSCCC) purification. As a result, the optimum transformation pH, temperature, and time were pH 4.0, 60 °C, and 60 min, respectively. The Km and Vmax were 0.36 mM and 0.460 mmol/min, respectively. For isoquercitrin production, the optimal rutin concentration and transformation time were approximately 1000 mg/L and 60 min. The rutin transformation rate reached 96.44%. The isoquercitrin was purified to a purity of 97.83% using one-step purification with HSCCC. The isoquercitrin was identified using UPLC-Q-TOF-MS. The comprehensive results indicated that the biotransformation procedure using the α-L-rhamnosidase from A. niger JMU-TS528 combined with HSCCC was a simple and effective process to prepare isoquercitrin, which might facilitate the production of isoquercitrin for industrial use.

Keywords: biotransformation; high-speed countercurrent chromatography; isoquercitrin; rutin; α-L-rhamnosidase.

MeSH terms

  • Aspergillus niger / metabolism*
  • Biotransformation
  • Countercurrent Distribution
  • Glycoside Hydrolases / metabolism*
  • Industrial Microbiology
  • Quercetin / analogs & derivatives*
  • Quercetin / isolation & purification
  • Quercetin / metabolism
  • Rutin / metabolism*


  • isoquercitrin
  • Rutin
  • Quercetin
  • Glycoside Hydrolases
  • alpha-L-rhamnosidase