Use of Human Intestinal Enteroids to Detect Human Norovirus Infectivity

Emerg Infect Dis. 2019 Sep;25(9):1730-1735. doi: 10.3201/eid2509.190205.


Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney-infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.

Keywords: Ct cutoff; Cycle threshold; Hong Kong; enteric infections; enteroids; human norovirus; infectivity; rRT-PCR; real-time reverse transcription PCR; viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Caliciviridae Infections / epidemiology*
  • Caliciviridae Infections / virology
  • China / epidemiology
  • Feces / virology
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Real-Time Polymerase Chain Reaction
  • Sensitivity and Specificity