Ozone and pulsed electro-magnetic field therapies improve endometrial lining thickness in frozen embryo transfer cycles: Three case reports

Medicine (Baltimore). 2019 Aug;98(34):e16865. doi: 10.1097/MD.0000000000016865.


Rationale: In assisted reproductive technology, a persistently thin endometrial lining represents a huge challenge during frozen embryo transfer (FET) cycles.

Patient concerns: Three patients who had a persistently thin endometrial lining despite the use of several medical agents known to improve endometrial lining thickness.

Diagnoses: Infertility undergoing FET cycles.

Interventions: A combination of transdermal and intravaginal ozone therapy along with Pulsed Electro-Magnetic Field (PEMF) therapy.

Outcomes: Ozone with PEMF, both of which are known to have vasodilatatory, anti-inflammatory, and anti-oxidant actions, were successful in improving the thickness of the endometrial lining in all 3 patients. Two out of 3 patients became pregnant following single embryo transfer.

Lessons: Ozone with PEMF constitute a novel experimental approach for women with persistently thin endometrial lining undergoing FET. This novel approach needs validation by large well-designed studies.

Publication types

  • Case Reports

MeSH terms

  • Administration, Cutaneous
  • Administration, Intravaginal
  • Adult
  • Cryopreservation
  • Embryo Transfer / methods
  • Endometrium / drug effects*
  • Endometrium / pathology
  • Female
  • Humans
  • Magnetic Field Therapy*
  • Oxidants, Photochemical / administration & dosage*
  • Oxidants, Photochemical / pharmacology
  • Ozone / administration & dosage*
  • Ozone / pharmacology
  • Pregnancy


  • Oxidants, Photochemical
  • Ozone