The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis

Arch Oral Biol. 2019 Nov:107:104513. doi: 10.1016/j.archoralbio.2019.104513. Epub 2019 Aug 10.


Objective: The aim of this study was to explore the role of macrophage migration inhibitory factor (MIF) and intercellular adhesion molecule (ICAM)-1 in rats with periodontitis and atherosclerosis.

Methods: Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups: control group (C), periodontitis group (P), atherosclerosis group (AS), and periodontitis plus atherosclerosis group (P + AS). The levels of MIF and ICAM-1 in serum were detected by an enzyme-linked immunosorbent assay (ELISA). The protein expression of MIF and ICAM-1 in the carotid artery tissues was examined by immunohistochemical staining.

Results: The results of the ELISA showed that the serum level of MIF in the P + AS group (59.40 ± 3.92 ng/mL) was significantly higher than that in the C group (42.93 ± 2.63 ng/mL), the P group (45.57 ± 2.59 ng/mL) and the AS group (50.88 ± 4.20 ng/mL) (P < 0.05). Similarly, the ICAM-1 level in the P + AS group (6.77 ± 1.47 ng/mL) was much higher than that in the C (1.33 ± 0.25 ng/mL), P (3.99 ± 0.44 ng/mL) and AS groups (4.19 ± 0.89 ng/mL) (P < 0.05). Furthermore, the expression of MIF, as assessed by immunohistochemical staining, was significantly higher in the artery tissues of the P + AS group than in the tissues of the other three groups (P < 0.05). However, there was no significant difference in the protein level of ICAM-1 between the P + AS and AS groups, where its expression was much stronger than in the C and the P groups.

Conclusion: Our results indicate that there is a close association between periodontitis and atherosclerosis. MIF and ICAM-1 may play a role in the development of atherosclerosis and periodontitis.

Keywords: Atherosclerosis; ICAM-1; MIF; Periodontitis; Rat.

MeSH terms

  • Animals
  • Atherosclerosis / metabolism*
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Intramolecular Oxidoreductases / metabolism*
  • Macrophage Migration-Inhibitory Factors / metabolism*
  • Male
  • Periodontitis / metabolism*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley


  • ICAM1 protein, rat
  • Macrophage Migration-Inhibitory Factors
  • Intercellular Adhesion Molecule-1
  • Intramolecular Oxidoreductases
  • Mif protein, rat