Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 19 (1), 230

Glucose-lowering and Hypolipidemic Activities of Polysaccharides From Cordyceps Taii in Streptozotocin-Induced Diabetic Mice

Affiliations

Glucose-lowering and Hypolipidemic Activities of Polysaccharides From Cordyceps Taii in Streptozotocin-Induced Diabetic Mice

Ru-Ming Liu et al. BMC Complement Altern Med.

Abstract

Background: Hyperglycemia and dyslipidemia are classic features of patients with diabetes mellitus (DM). Cordyceps taii, a folk medicinal fungus native to southern China, possesses various pharmacological activities. This study aimed to assess the glucose-lowering and hypolipidemic effects of polysaccharides from C. taii (CTP) in streptozotocin (STZ)-induced diabetic mice.

Methods: Kunming mice were intraperitoneally injected with STZ at a dose of 100 mg/kg body weight. After induction of diabetes, diabetic mice were randomly divided into five groups: diabetic mellitus group (DM), metformin-treated group, low, medium, and high-dose CTP-treated group (CTP-L, CTP-M, and CTP-H). Normal mice served as the control group. After treatment for 28 days, body weight, fasting serum insulin (FSI), fasting blood glucose (FBG), homeostasis model assessment-insulin resistance (HOMA-IR), triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP) levels were measured. Histological analysis of pancreatic tissue and immune organ indices was also performed to evaluate the anti-diabetes effect of CTP. SPSS (version 21.0) software was used for statistical analysis, and statistical differences were considered significant at p < 0.05.

Results: Compared with the DM group, the body weight and FSI level of CTP-H group increased by 36.13 and 32.47%, whereas the FBG and HOMA-IR decreased by 56.79 and 42.78%, respectively (p < 0.05). Histopathological examination of the pancreas revealed that CTP improved and repaired the impaired islet β-cells in pancreatic tissue. Compared with the DM group, the levels of TC, TG, and LDL-C decreased by 13.84, 31.87, and 36.61%, whereas that of HDL-C increased by 28.60% in CTP-H (p < 0.05). Further study showed that the thymus index in CTP-H was elevated by approximately 54.96%, and the secretion of pro-inflammatory cytokines TNF-α, IL-6, and CRP was inhibited by approximately 19.97, 34.46, and 35.41%, respectively (p < 0.05).

Conclusion: The anti-diabetes effect of CTP is closely associated with immunoregulation and anti-inflammation, and CTP may be considered as a therapeutic drug or functional food for DM intervention.

Keywords: Cordyceps taii; Diabetes mellitus; Glucose-lowering effect; Hypolipidemia; Polysaccharides.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Schematic of the treatment schedule. Adult male Kunming mice (6 weeks old) underwent adapted feeding for 7 days. Except in the CON group, all of the mice were ip injected with 100 mg/kg bw STZ (dissolved in an ice-cold 0.1 mol/L sodium citrate buffer) once. DM mice (fasting blood glucose concentrations higher than 11.1 mmol/L) were randomly divided into five groups after 3 days of modeling: diabetes mellitus group (DM), metformin-treated group (MET), and CTP (CTP-L, CTP-M and CTP-H) group. All of the mice were dosed by gavage once daily for 28 consecutive days
Fig. 2
Fig. 2
Effects of CTP on body weight, FBG, insulin level, and HOMA-IR value in STZ-induced diabetic mice. a Body weight was measured at regular intervals after CTP treatment. b FBG level was measured at regular intervals after CTP treatment. c Insulin level was measured 28 days after CTP was administered. d HOMA-IR value was estimated 28 days after CTP was administered. Data are presented as mean ± SD (n = 8–12). * p < 0.05 as compared with the normal control group (CON). # p < 0.05 as compared with DM
Fig. 3
Fig. 3
Effects of CTP on serum lipid levels in diabetic mice. The following values were measured 28 days after CTP was administered: (a) Serum TC level. b Serum TG level. c Serum LDL-C level. d Serum HDL-C level. Data are presented as mean ± SD (n = 8–12). * p < 0.05 as compared with CON. # p < 0.05 as compared with DM
Fig. 4
Fig. 4
HE staining showing the histopathological changes of the pancreas in diabetic mice treated with CTP for 28 days (200× magnification). The representative data from three independent experiments are shown
Fig. 5
Fig. 5
Effects of CTP on immune organ indices in diabetic mice. The following indices were measured 28 days after CTP was administered (a) Spleen index. b Thymus index. Data are presented as the mean ± SD (n = 8–12). * p < 0.05 as compared with CON. # p < 0.05 as compared with DM
Fig. 6
Fig. 6
Effects of CTP on the serum inflammatory cytokine levels in diabetic mice. The following values were measured 28 days after CTP was administered (a) TNF-α levels. b IL-6 levels. c CRP levels. Data are presented as the mean ± SD (n = 8–12). * p < 0.05 as compared with CON. # p < 0.05 as compared with DM

Similar articles

See all similar articles

References

    1. Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol. 2018;14:88–98. doi: 10.1038/nrendo.2017.151. - DOI - PubMed
    1. International Diabetes Federation. IDF Diabetes Atlas, 8th ed., International Diabetes Federation, Brussels, Belgium, 2017.
    1. Bannister M, Berlanga J. Effective utilization of oral hypoglycemic agents to achieve individualized HbA1c targets in patients with type 2 diabetes mellitus. Diabetes ther. 2016;7:387–399. doi: 10.1007/s13300-016-0188-5. - DOI - PMC - PubMed
    1. Scheen AJ. Drug interactions of clinical importance with antihyperglycaemic agents: an update. Drug Saf. 2005;28:601–631. doi: 10.2165/00002018-200528070-00004. - DOI - PubMed
    1. Das B, Satyalakshmi G. Natural products based anticancer agents. Mini-Rev Org Chem. 2012;9:169–177. doi: 10.2174/157019312800604706. - DOI
Feedback