Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity

Nisreen Shumayrikh; Dipankar Sen

doi:10.1007/978-1-4939-9666-7_22

Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity

Methods Mol Biol. 2019:2035:357-368. doi: 10.1007/978-1-4939-9666-7_22.

Authors

Nisreen Shumayrikh¹, Dipankar Sen^{2

3}

Affiliations

¹ Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
² Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada. sen@sfu.ca.
³ Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada. sen@sfu.ca.

PMID: 31444762
DOI: 10.1007/978-1-4939-9666-7_22

Abstract

Catalytic DNAs (DNAzymes) with peroxidase-like activity have great potential in bioanalytical chemistry [1], owing to numerous advantages that DNA enzymes offer over conventional protein enzymes, including structural simplicity, low cost, thermal stability, and straightforward handling and preparation. Maximizing the efficiency of the peroxidase activity of such DNAzymes is a subject in need of review. In this chapter, we discuss the optimal experimental conditions for the peroxidase activity of these DNAzymes and describe general procedures for their utilization.

Keywords: ABTS; Amplex Red; DNAzyme; Guanine quadruplex; HRP; Heme; Hemin; Peroxidase activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques
DNA, Catalytic / chemistry*
G-Quadruplexes*
Peroxidases / metabolism*

Substances

DNA, Catalytic
Peroxidases