Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity

Methods Mol Biol. 2019:2035:357-368. doi: 10.1007/978-1-4939-9666-7_22.


Catalytic DNAs (DNAzymes) with peroxidase-like activity have great potential in bioanalytical chemistry [1], owing to numerous advantages that DNA enzymes offer over conventional protein enzymes, including structural simplicity, low cost, thermal stability, and straightforward handling and preparation. Maximizing the efficiency of the peroxidase activity of such DNAzymes is a subject in need of review. In this chapter, we discuss the optimal experimental conditions for the peroxidase activity of these DNAzymes and describe general procedures for their utilization.

Keywords: ABTS; Amplex Red; DNAzyme; Guanine quadruplex; HRP; Heme; Hemin; Peroxidase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • DNA, Catalytic / chemistry*
  • G-Quadruplexes*
  • Peroxidases / metabolism*


  • DNA, Catalytic
  • Peroxidases