A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1

Genes Dev. 1988 Oct;2(10):1216-26. doi: 10.1101/gad.2.10.1216.

Abstract

ATF is a cellular transcription factor involved in the regulation of multiple adenovirus E1A- and cellular cAMP-inducible promoters. Using DNA affinity chromatography, we have purified ATF and found that a series of polypeptides copurify in a sequence-specific manner. We demonstrate that these polypeptides represent a family of proteins that are related by DNA-binding specificity and by immunological cross-reactivity. This family includes the transcription factor AP-1, whose recognition sequence, GTGAGTCAA, differs from the ATF consensus, GTGACGTCAA, by the absence of a cytosine residue. Our results further indicate that there are multiple forms of both ATF and AP-1. The immunological cross-reactivity and related DNA-binding specificities suggest that ATF and AP-1 contain similar amino acid sequences and may have originated from a common gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activating Transcription Factors
  • Blood Proteins*
  • Centrifugation, Density Gradient
  • Chromatography, Affinity
  • Cross Reactions
  • DNA-Binding Proteins / immunology*
  • DNA-Binding Proteins / metabolism
  • Deoxyribonuclease I
  • Humans
  • Molecular Weight
  • Peptide Fragments / immunology
  • Peptide Fragments / isolation & purification
  • Proto-Oncogene Proteins c-jun
  • Transcription Factors / immunology*
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism

Substances

  • Activating Transcription Factors
  • Blood Proteins
  • DNA-Binding Proteins
  • Peptide Fragments
  • Proto-Oncogene Proteins c-jun
  • Transcription Factors
  • Deoxyribonuclease I