Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Stem Cell Res. 2019 Oct;40:101533. doi: 10.1016/j.scr.2019.101533. Epub 2019 Aug 9.

Abstract

Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Cell Differentiation
  • Cell Line
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism
  • Exons
  • Gene Editing*
  • Humans
  • Karyotype
  • Methyl-CpG-Binding Protein 2 / genetics*
  • Rett Syndrome / genetics
  • Rett Syndrome / pathology

Substances

  • Methyl-CpG-Binding Protein 2