In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds

Methods Mol Biol. 2020;2052:335-350. doi: 10.1007/978-1-4939-9748-0_19.

Abstract

Hollow fiber technology is a powerful tool for the culture of difficult-to-grow cells. Cryptosporidium parvum has a multistage sexual and asexual life cycle that has proved difficult to culture by conventional in vitro culture methods. Here, we describe a method utilizing a hollow fiber bioreactor for the continuous in vitro growth of C. parvum that produces sexual and asexual stages. The method enables the evaluation of potential therapeutic compounds under conditions that mirror the dynamic conditions found in the gut facilitating preliminary pharmacokinetic and pharmacodynamic data to be obtained.

Keywords: Cryptosporidium parvum; Hollow fiber bioreactor; In vitro culture; Pharmacodynamics; Pharmacokinetic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiprotozoal Agents / pharmacokinetics*
  • Antiprotozoal Agents / pharmacology*
  • Bioreactors / parasitology*
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods*
  • Cell Line, Tumor
  • Cryptosporidium parvum / drug effects*
  • Cryptosporidium parvum / growth & development
  • Cryptosporidium parvum / metabolism
  • Humans
  • Oocysts / drug effects*
  • Oocysts / growth & development
  • Oocysts / isolation & purification
  • Oocysts / metabolism
  • Workflow

Substances

  • Antiprotozoal Agents