doi: 10.1016/0161-5890(88)90005-3.
Hybridoma fusion cell lines contain an aberrant kappa transcript
Affiliations
- PMID: 3146025
- DOI: 10.1016/0161-5890(88)90005-3
Item in Clipboard
Hybridoma fusion cell lines contain an aberrant kappa transcript
Mol Immunol.
1988 Oct.
Abstract
The V region sequence of a non-productive kappa transcript from two myeloma fusion partners has been determined. This transcript has an aberrant VJ recombination site resulting in a translation stop site at position 105. It is variably expressed in hybridomas made from all fusion partners derived from the original MOPC-21 tumor. The amount of this transcript may greatly exceed levels of the productive light chain mRNA.
Similar articles
-
Selective PCR amplification of functional immunoglobulin light chain from hybridoma containing the aberrant MOPC 21-derived V kappa by PNA-mediated PCR clamping.Biotechniques. 1999 May;26(5):818-20, 822. doi: 10.2144/99265bm04. Biotechniques. 1999. PMID: 10337468 No abstract available.
-
Purification of mRNA for immunoglobulin kappa-chains from myeloma and hybridoma cells using hybridization to immobilized complementary DNA.Immunol Lett. 1984;7(6):315-9. doi: 10.1016/0165-2478(84)90087-7. Immunol Lett. 1984. PMID: 6427101
-
An improved method for generating single-chain antibodies from hybridomas.J Immunol Methods. 1993 Sep 27;165(1):81-91. doi: 10.1016/0022-1759(93)90109-k. J Immunol Methods. 1993. PMID: 8409471
-
Expression, allelic exclusion and somatic mutation of mouse immunoglobulin kappa genes.Immunol Rev. 1986 Feb;89:85-102. doi: 10.1111/j.1600-065x.1986.tb01474.x. Immunol Rev. 1986. PMID: 3081435 Review. No abstract available.
-
Spontaneous immunoglobulin class switching in myeloma and hybridoma cell lines differs from physiological class switching.Immunol Rev. 1982;67:59-72. doi: 10.1111/j.1600-065x.1982.tb01055.x. Immunol Rev. 1982. PMID: 6751996 Review. No abstract available.
Cited by
-
Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody.Malar J. 2015 Feb 5;14:50. doi: 10.1186/s12936-015-0577-7. Malar J. 2015. PMID: 25651860 Free PMC article.
-
Molecular characterization of a fully human chimeric T-cell antigen receptor for tumor-associated antigen EpCAM.J Biomed Biotechnol. 2012;2012:853879. doi: 10.1155/2012/853879. Epub 2012 Apr 3. J Biomed Biotechnol. 2012. PMID: 22547929 Free PMC article.
-
NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research.bioRxiv [Preprint]. 2023 Jun 30:2023.06.28.546392. doi: 10.1101/2023.06.28.546392. bioRxiv. 2023. Update in: Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4 PMID: 37425915 Free PMC article. Updated. Preprint.
-
Hybridoma passage in vitro may result in reduced ability of antimannan antibody to protect against disseminated candidiasis.Infect Immun. 2006 Jul;74(7):4310-21. doi: 10.1128/IAI.00234-06. Infect Immun. 2006. PMID: 16790805 Free PMC article.
-
A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.PLoS One. 2021 Oct 11;16(10):e0252558. doi: 10.1371/journal.pone.0252558. eCollection 2021. PLoS One. 2021. PMID: 34634047 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources