A novel device to prevent osteoporosis by promoting bone metabolism using a newly developed double-loading stimulation with vibration and shaking

Runhong Yao; Kazuhiro Nishii; Takumi Kito; Toshio Teranishi; Tomohisa Sugiyama; Kazuyoshi Sakai; Mamoru Matsubara; Kouji Yamada

doi:10.2535/ofaj.96.13

A novel device to prevent osteoporosis by promoting bone metabolism using a newly developed double-loading stimulation with vibration and shaking

Okajimas Folia Anat Jpn. 2019;96(1):13-21. doi: 10.2535/ofaj.96.13.

Authors

Runhong Yao¹, Kazuhiro Nishii¹, Takumi Kito¹, Toshio Teranishi¹, Tomohisa Sugiyama¹, Kazuyoshi Sakai¹, Mamoru Matsubara², Kouji Yamada¹

Affiliations

¹ Graduate School of Health Sciences, Fujita Health University.
² Department of Bioscience and Biotechnology, Kyoto Gakuen University.

PMID: 31462620
DOI: 10.2535/ofaj.96.13

Abstract

In Japan, 13 million people have osteoporosis, including approximately 9 hundred thousand people who are bedridden owing to bone fractures from falls. Preventing osteoporosis is considered to be an important and effective way of preventing fall-related fractures. Thus, we developed a novel method of locomotor stimulation and analyzed its effectiveness in mice. Specifically, we created a double-loading device that combines vibration and shaking stimulation. The device was used to continuously stimulate ovariectomy-induced decreased bone density mouse models 30 minutes daily for 10 weeks. We then collected femur samples, created undecalcified tissue slices, calculated parameters using bone histomorphomtry, and conducted comparative testing. BS/TV (bone surface/tissue volume), N.Oc/ES (osteoclast number/eroded surface), Oc.S/ES (osteoclast osteoid surface/eroded surface), Omt (osteoid maturation time), Tb.N (trabecular number), Mlt (mineralization lag time) < (p < 0.01), N.Ob (osteoblast number), N.Ob/TV (osteoblast number/tissue volume), sLS (single labeled suface), N.Mu.Oc/ES (multinucle osteoclast number/eroded surface), and N.Mo.Oc/ES (mononucle osteoclast number/eroded surface) (p < 0.05) were significantly higher in the stimulation group than in the non-stimulation group. In addition, BS/BV (bone surface/bone volume), Tb.Sp (trabecular separation), MAR (mineral apposition rate), Aj.Ar (adjusted apposition rate) (p < 0.01), ES (eroded surface ), ES/BS (eroded surface/bone surface), and BRs.R (bone resorption rate) (p < 0.05) were significantly lower in the stimulation group than in the non-stimulation group. These results suggest that stimulation activated osteoblasts and osteoclasts, thereby leading to highly active bone remodeling. We anticipate that bone mineralization will subsequently occur, suggesting that this stimulation technique is effective in preventing osteoporosis by alleviating sudden bone density loss.

Keywords: Bone mineral density; Osteoporosis; Postmenopausal women; Prevention; Vibration and shaking.

MeSH terms

Animals
Bone and Bones / metabolism*
Female
Mice, Inbred ICR
Osteoporosis / prevention & control*
Vibration