Neonates with suspected microangiopathic disorders: performance of standard manual schistocyte enumeration vs. the automated fragmented red cell count

J Perinatol. 2019 Nov;39(11):1555-1561. doi: 10.1038/s41372-019-0482-y. Epub 2019 Aug 28.

Abstract

Objectives: To enhance the diagnosis of schistocyte-producing conditions, we compared routine manual schistocyte enumeration with automated fragmented red cell counts (FRCs).

Study design: In neonates "suspected" of having sepsis, NEC, or DIC we compared manual schistocyte estimates vs. automated FRC counts. When the two disagreed, we used a "gold standard" from a ≥ 1000 RBC differential. We also assessed the diagnostic accuracy of the FRC count in diagnosing sepsis, NEC, or DIC.

Results: We collected 270 CBCs from 90 neonates. The methods agreed in 63% (95% CI 55%-70%) of the CBCs. Among the 37% where they disagreed, the FRC count was more accurate in 100% (95% CI 88-100%). An elevated FRC count was specific for sepsis, and was sensitive and specific for necrotizing enterocolitis and DIC.

Conclusions: Automated FRC counts have advantages over routine manual evaluation, larger sample size, lower expense, and superior accuracy in diagnosing schistocyte-producing conditions.

Publication types

  • Comparative Study
  • Multicenter Study

MeSH terms

  • Automation, Laboratory*
  • Erythrocyte Count / instrumentation*
  • Erythrocyte Count / methods*
  • Erythrocytes, Abnormal / cytology*
  • Female
  • Humans
  • Infant, Newborn
  • Intensive Care Units, Neonatal
  • Male
  • Reference Values
  • Thrombotic Microangiopathies / blood
  • Thrombotic Microangiopathies / diagnosis*
  • Utah