Expression Analysis by RNAscope™ In Situ Hybridization

Timothy J Mead; Suneel S Apte

doi:10.1007/978-1-4939-9698-8_14

Expression Analysis by RNAscope™ In Situ Hybridization

Methods Mol Biol. 2020:2043:173-178. doi: 10.1007/978-1-4939-9698-8_14.

Authors

Timothy J Mead¹, Suneel S Apte²

Affiliations

¹ Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.
² Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA. aptes@ccf.org.

PMID: 31463911
DOI: 10.1007/978-1-4939-9698-8_14

Abstract

RNA in situ hybridization has an important place in matrix biology, as the only method that allows for in situ discrimination of precise spatial and temporal patterns of gene expression. Whereas immunohistochemistry shows where a matrix protein localizes, ISH identifies the cell of origin. Thus, these methods provide complementary information for insights on the life cycle of matrix molecules, including ADAMTS proteases. This protocol encompasses the staining of tissue sections to reveal expression of the gene of interest.

Keywords: Gene expression; In situ; RNA; Tissue.

MeSH terms

ADAMTS Proteins / genetics*
Animals
Gene Expression
In Situ Hybridization / methods*
Indicators and Reagents
Mice
RNA, Messenger / genetics

Substances

Indicators and Reagents
RNA, Messenger
ADAMTS Proteins