Visualization and Quantification of Pericellular Matrix

Timothy J Mead; Suneel S Apte

doi:10.1007/978-1-4939-9698-8_21

Visualization and Quantification of Pericellular Matrix

Methods Mol Biol. 2020:2043:261-264. doi: 10.1007/978-1-4939-9698-8_21.

Authors

Timothy J Mead¹, Suneel S Apte²

Affiliations

¹ Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.
² Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA. aptes@ccf.org.

PMID: 31463918
DOI: 10.1007/978-1-4939-9698-8_21

Abstract

The pericellular matrix (PCM), also known as the pericellular coat or glycocalyx, lies between the plasma membrane and the interstitial extracellular matrix (ECM). It can have a dramatic influence on cell function because of its presence at the interface between the cell and its microenvironment. A common tool used to demonstrate the PCM is the particle exclusion assay in which fixed red blood cells are utilized to outline the boundary of the cell together with its PCM. PCM visualization and quantification provide opportunities to uncover the roles of ADAMTS proteases in PCM remodeling in many cell types and processes.

Keywords: Erythrocyte; Glycocalyx; Particle exclusion; Pericellular matrix; Red blood cell.

MeSH terms

Cell Membrane / ultrastructure*
Cells, Cultured
Extracellular Matrix / ultrastructure*
Humans
Male
Microscopy, Fluorescence
Myocytes, Smooth Muscle / ultrastructure*
Software