Genotoxic action of Luna Experience-SC 400 fungicide on rat bone marrow

Biomarkers. 2019 Nov;24(7):720-725. doi: 10.1080/1354750X.2019.1658804. Epub 2019 Aug 29.


Background: Fungicides describe all chemicals used to control fungi that infect plants. Luna Experience SC-400 is a new line of fungicide that consist of Fluopyram and Tebuconazole. Objective: In this study, We investigated the genotoxicty and cytotoxicty of Luna Experience-SC 400 using comet assay, micronucleus test and polychromatic erythrocytes number in rat bone marrow. The present study is the first report indicating the effects of genotoxic and cytotoxic of Luna experience SC-400 on rat bone marrow cells. Material and Methods: We used three different doses (5mg/kg, 10mg/kg, 20mg/kg) of Luna Experience SC 400 at 48 h intervals during 30 days by gavage in rats.Genotoxicity was evaluated using comet assay and micronucleus test and cytotoxicity was measured the PCE/NCE rate in rat bone marrow. Results: Based on these experimental results, we report that Luna Experience-SC 400 fungicide presents genotoxic and cytotoxic potential on rat bone marrow. There is a significant difference between negative control group and all the doses of Luna Experience-SC 400 (p < 0.05) for comet assay and micronucleus. Even moderate and high doses of fungicides seem to have reached the values of almost positive control group for Genetic Damage Index (GDI) and Damaged Cell Percentage (DCP). In this study, we also investigated the PCE/NCE rate. Fungicide caused a decrease in the level of significant in the PCE/NCE ratio (p < 0.05). Conclusion: Our in vivo study suggests that the gavage exposure to Luna experience SC 400 used in the present investigation may be genotoxic and cytotoxic in rat bone marrow in view of these findings. Because this findings is first report represented in the pesticide biology, it is important to carry out more investigations using various cytogenetic tests under different experimental conditions to definitively resolve the the possible genotoxic and cytotoxic risk associated with new generation pesticides-fungicides.

Keywords: Comet assay; DNA damage; bone marrow; cytotoxicity; fungicides (Luna Experience-SC 400); genotoxicity; micronucleus assay.

MeSH terms

  • Animals
  • Benzamides / toxicity*
  • Bone Marrow / drug effects*
  • Comet Assay
  • Cytotoxins / pharmacology
  • Dose-Response Relationship, Drug
  • Fungicides, Industrial / toxicity*
  • Micronucleus Tests
  • Mutagens / pharmacology
  • Pyridines / toxicity*
  • Rats
  • Triazoles / toxicity*


  • Benzamides
  • Cytotoxins
  • Fungicides, Industrial
  • Mutagens
  • Pyridines
  • Triazoles
  • tebuconazole
  • N-(2-(3-chloro-5-(trifluoromethyl)-2-pyridyl)ethyl)-alpha,alpha,alpha-trifluoro-o-toluamide