Specificity of proliferative response of human CD8 clones to mycobacterial antigens

Eur J Immunol. 1988 Dec;18(12):1881-7. doi: 10.1002/eji.1830181203.


Human CD8 T lymphocyte clones (TLC) were generated from the pleural effusion of patients with tuberculosis using a protocol that required, in addition to antigen, coculture of purified CD8+ T cells, accessory cells, interleukin 2 (IL2) and anti-CD3-Sepharose. The TLC obtained were stimulated by mycobacterial soluble extracts in an IL2-dependent and MHC class I-restricted manner. When antigen-responsive TLC were screened with extracts from the recombinant mycobacterial library they were found to respond to either the Y3125 (100-kDa) or the Y3111 (71-kDa) lambda gt11 clones. Polyacrylamide gel immunoblot analysis demonstrated that the CD8 TLC responded to fractions with the molecular mass range 27-45 kDa in the Y3125 lysogen and 60-90 kDa in the mycobacterial soluble extract. The specificity of TLC reactive with the Y3111 clone was confirmed using the 71-kDa antigen purified from the same lysogen. These TLC recognized sequences common to the 71-kDa protein derived from mycobacteria, E. coli or a human cell line. Studies of three TLC using antigen-presenting cells of known genetic haplotype indicated that stimulation with both the Y3125 and the 71-kDa antigens were restricted by determinants encoded by HLA-B8.

MeSH terms

  • Antigens, Bacterial / immunology*
  • Antigens, Differentiation, T-Lymphocyte / analysis*
  • CD8 Antigens
  • Clone Cells
  • Growth Substances / pharmacology
  • Histocompatibility Antigens Class I / immunology
  • Humans
  • In Vitro Techniques
  • Interleukin-2 / pharmacology
  • Interleukin-4
  • Interleukins / pharmacology
  • Lymphocyte Activation
  • Major Histocompatibility Complex
  • Molecular Weight
  • Mycobacterium / immunology*
  • Recombinant Proteins / immunology
  • T-Lymphocytes, Cytotoxic / immunology*


  • Antigens, Bacterial
  • Antigens, Differentiation, T-Lymphocyte
  • CD8 Antigens
  • Growth Substances
  • Histocompatibility Antigens Class I
  • Interleukin-2
  • Interleukins
  • Recombinant Proteins
  • Interleukin-4