Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells

Genomics. 2020 Mar;112(2):1490-1499. doi: 10.1016/j.ygeno.2019.08.020. Epub 2019 Aug 26.


In this study, we investigate the effect of one such micro RNA, miR-214 which is frequently down-regulated in cervical cancer. In this study, we either CRISPR knocked out or overexpressed miR-214 in cervical cancer cells and analyzed the global mRNA expression by Next Generation Sequencing (NGS) It was observed that a total of 108 genes were upregulated and 178 downregulated between the samples, above and below the baseline respectively. Gene Ontology and KEGG pathway analysis reveal distinct biological processes and pathways. Analysis of gene regulatory networks also gave different network patterns in the two samples. We confirmed the RNA sequencing data for 10 genes; IFIF27, SMAD3, COX11, TP53INP1, ABL2, FGF8, TNFAIP3, NRG1, SP3 and MDM4 by Real-time PCR. This is the first report on the effect of miR-214 on global mRNA profile in cervical cancer cells. This study also reports new biomarkers for cervical cancer prognosis.

Keywords: Cervical cancer; Network; RNA; Sequencing; Transcriptome; miR-214.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • CRISPR-Cas Systems
  • Cell Line, Tumor
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Gene Knockout Techniques
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Transcriptome*
  • Uterine Cervical Neoplasms / genetics*


  • Biomarkers, Tumor
  • MIRN124 microRNA, human
  • MicroRNAs