Maternal manganese activates anti-apoptotic-related gene expressions via miR-1551 and miR-34c in embryonic hearts from maternal heat stress (Gallus gallus)

J Therm Biol. 2019 Aug:84:190-199. doi: 10.1016/j.jtherbio.2019.07.014. Epub 2019 Jul 3.


MicroRNAs (miRNAs) expressions are altered by maternal stresses and nutritional status. Our previous study has demonstrated that maternal manganese (Mn) addition could protect chick embryos against maternal heat stress via enhancing anti-apoptotic ability in embryonic hearts. The objective of this study was to investigate whether this protective effect could be achieved via miRNA mechanisms, and also be sustained in offspring broilers. A completely randomized design with a 2 (maternal normal and high temperatures: 21 and 32 °C) × 2 (maternal control basal diet and the basal diet + 120 mg Mn/kg) factorial arrangement of treatments was adopted. Totally 96 broiler breeder hens were allotted to 4 treatments with 6 replicates. Subsequently, 24 hatched chicks from each maternal treatment were divided into 6 replicates. Maternal supplemental 120 mg Mn/kg reduced the increased expressions of miR-1551 and miR-34c in hearts of offspring embryos but not broilers under maternal heat stress. B-cell CLL/lymphoma 2 (BCL2) and NF-κB-inducing kinase (NIK) genes related to anti-apoptotic ability were identified as direct targets for miR-1551 and miR-34c, respectively. Under maternal heat stress, maternal supplemental 120 mg Mn/kg activated target BCL2 expression and NIK-dependent NF-κB pathway via mediating miR-1551 and miR-34c expressions in hearts of offspring embryos rather than broilers.

Keywords: Anti-apoptotic gene expression; Heart; Manganese; Maternal heat stress; MicroRNA expression; Offspring chick embryo.

Publication types

  • Randomized Controlled Trial, Veterinary

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Avian Proteins / genetics
  • Bird Diseases*
  • Chick Embryo
  • Chickens
  • Female
  • Gene Expression Regulation, Developmental / drug effects*
  • Heart / drug effects*
  • Heart / embryology
  • Heat Stress Disorders / veterinary*
  • Male
  • Manganese / pharmacology*
  • MicroRNAs
  • NF-kappa B / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics


  • Avian Proteins
  • MicroRNAs
  • NF-kappa B
  • Proto-Oncogene Proteins c-bcl-2
  • Manganese