Taurine-Rich-Containing Hot Water Extract of Loliolus Beka Gray Meat Scavenges Palmitate-Induced Free Radicals and Protects Against DNA Damage in Insulin Secreting β-Cells

Adv Exp Med Biol. 2019:1155:483-495. doi: 10.1007/978-981-13-8023-5_45.

Abstract

The loss of pancreatic β-cells plays a central role in the pathogenesis of both type 1 and type 2 diabetes, and many studies have been focused on ways to improve glucose homeostasis by preserving, expanding and improving the function of β-cell. Elevated levels of free fatty acids such as palmitate might contribute to the loss of β-cells. A marine squid, Loliolus beka has long been used as a food in Korea, China, Japan and Europe due to its tender meat and high taurine content. Here, we investigated the protective effects of a hot water extract of Loliolus beka meat (LBM) against palmitate toxicity in Ins-1 cells, a rat β-cell line. Treatment with LBM extract protected against palmitate-induced cytotoxicity and scavenged overproduction of nitric oxide, alkyl, and hydroxyl radicals. In addition, LBM extract protected against palmitate-induced DNA damage and β-cell dysfunction. These findings suggest that LBM protects pancreatic β-cells from palmitate-induced damage. LBM could be a potential therapeutic functional food for diabetes.

Keywords: Beta-cell; Diabetes; Loliolus beka; Loliolus beka meat; Seaweed; Taurine.

MeSH terms

  • Animals
  • Apoptosis
  • Cell Extracts / pharmacology*
  • Cell Line
  • DNA Damage*
  • Decapodiformes / chemistry*
  • Diabetes Mellitus
  • Free Radicals / adverse effects*
  • Insulin-Secreting Cells / drug effects*
  • Meat
  • Palmitates / adverse effects*
  • Rats
  • Taurine / pharmacology*

Substances

  • Cell Extracts
  • Free Radicals
  • Palmitates
  • Taurine