Purpose: Sleep is considered essential for muscle recovery, mainly due to its effect on hormone secretion. Total sleep deprivation or restriction is known to alter not only blood hormones but also cytokines that might be related to skeletal muscle recovery. This study aimed to evaluate whether total sleep deprivation after eccentric exercise-induced muscle damage (EEIMD) modifies the profiles of blood hormones and cytokines.
Methods: In two separate conditions, with a crossover and randomized model, 10 men (age, 24.5 ± 2.9 yr; body mass index, 22.7 ± 2.3 kg·m) performed a unilateral EEIMD protocol that comprised 240 eccentric contractions of the knee extensor muscles using an isokinetic dynamometer. In one condition, a "muscle damage" protocol was followed by 48 h of total sleep deprivation and 12 h of normal sleep (DEPRIVATION). In the other condition, the same muscle damage protocol was conducted, followed by three nights of regular sleep (SLEEP). Isometric muscle voluntary contraction tests and blood samples were collected serially throughout the protocol and analyzed for creatine kinase, free and total testosterone, IGF-1, cortisol, tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, receptor antagonist of IL-1 and IL-10.
Results: Muscle voluntary contraction and serum creatine kinase increased equally over the study period in both conditions. From the cytokines evaluated, only IL-6 increased in DEPRIVATION. No differences were detected in testosterone levels between conditions, but IGF-1, cortisol, and cortisol/total testosterone ratio were higher in DEPRIVATION.
Conclusions: Total sleep deprivation after EEIMD does not delay muscle strength recovery but modifies inflammatory and hormonal responses.
Effect of acute sleep deprivation and recovery on Insulin-like Growth Factor-I responses and inflammatory gene expression in healthy men.Eur Cytokine Netw. 2014 Jul-Sep;25(3):52-7. doi: 10.1684/ecn.2014.0356. Eur Cytokine Netw. 2014. PMID: 25373853
Effects of partial sleep deprivation on proinflammatory cytokines, growth hormone, and steroid hormone concentrations during repeated brief sprint interval exercise.Chronobiol Int. 2013 May;30(4):502-9. doi: 10.3109/07420528.2012.742102. Epub 2013 Jan 2. Chronobiol Int. 2013. PMID: 23281720
Neuromuscular Changes and Damage after Isoload versus Isokinetic Eccentric Exercise.Med Sci Sports Exerc. 2016 Dec;48(12):2526-2535. doi: 10.1249/MSS.0000000000001042. Med Sci Sports Exerc. 2016. PMID: 27434079
Induction and Assessment of Exertional Skeletal Muscle Damage in Humans.J Vis Exp. 2016 Dec 11;(118):54859. doi: 10.3791/54859. J Vis Exp. 2016. PMID: 28060273 Free PMC article.
Effects of recovery sleep after one work week of mild sleep restriction on interleukin-6 and cortisol secretion and daytime sleepiness and performance.Am J Physiol Endocrinol Metab. 2013 Oct 1;305(7):E890-6. doi: 10.1152/ajpendo.00301.2013. Epub 2013 Aug 13. Am J Physiol Endocrinol Metab. 2013. PMID: 23941878 Free PMC article.
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