Paper-based point-of-care immunoassays: Recent advances and emerging trends

Fei Li; Minli You; Shaoxiong Li; Jie Hu; Chang Liu; Yan Gong; Huayuan Yang; Feng Xu

doi:10.1016/j.biotechadv.2019.107442

Paper-based point-of-care immunoassays: Recent advances and emerging trends

Biotechnol Adv. 2020 Mar-Apr:39:107442. doi: 10.1016/j.biotechadv.2019.107442. Epub 2019 Aug 27.

Authors

Fei Li¹, Minli You¹, Shaoxiong Li², Jie Hu³, Chang Liu¹, Yan Gong³, Huayuan Yang⁴, Feng Xu⁵

Affiliations

¹ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
² The Engineering and Application Laboratory of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China; Center of Chinese Medicine Engineering, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
³ Diyinan Biotech Company, Suzhou 215000, PR China.
⁴ The Engineering and Application Laboratory of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China; Center of Chinese Medicine Engineering, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China. Electronic address: yhy4@shutcm.edu.cn.
⁵ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: fengxu@mail.xjtu.edu.cn.

PMID: 31470046
DOI: 10.1016/j.biotechadv.2019.107442

Abstract

Immunoassay has been routinely used in hospitals and central labs. Nowadays, to further meet the requirement of widespread applications of immunoassays, it is urgently needed to produce a simplified, rapid and low-cost immunoassay to perform tests on site. To this end, paper-based point-of-care (POC) immunoassays have attracted intensive interests in recent years. In this paper, we present a comprehensive review of the recent advances and emerging trends of paper-based POC immunoassays, including the fundamental components and work principles, various detection mechanisms and applications, and existing commercialized devices/products. At last, we envision three promising development directions for paper-based POC immunoassays.

Keywords: Immunoassays; Lateral flow immunoassay (LFIA); Microfluidic paper-based analytical devices (μPADs); Nanoparticles; Point-of-care testing (POCT).

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Immunoassay
Point-of-Care Systems*