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, 33 (5), 1447-1454

Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

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Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

Lara Schorn et al. In Vivo.

Abstract

Background/aim: Results of Guided Bone Regeneration (GBR) primarily depend on the membrane used. The aim of this study was to compare biocompatibility of different absorbable and non-absorbable membranes by using unrestricted somatic stem cells (USSCs) as an indicator for biocompatibility.

Materials and methods: Five absorbable membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend® and BioMend® Extend™) and one non-absorbable alternative (GORE-TEX®) were colonized with USSCs. After 24 h, 3 days and 7 days, cell proliferation, cell viability, and cytotoxicity were assessed. Moreover, cell morphology was evaluated by electron microscopy.

Results: Significantly higher cell proliferation and cell viability rates were observed in Bio-Gide® and RESODONT® membranes. Cell toxicity was highest on GENTA-FOIL resorb® membranes. The electron microscopical assessment showed a better cell attachment on porous surfaced membranes.

Conclusion: This study shows that USSCs can be used for assessments of biocompatibility, and that absorbable membranes with collagenous composition and porous structure tend to positively impact biocompatibility and enhance cell proliferation.

Keywords: Biocompatibility; guided bone regeneration (GBR); membranes; unrestricted somatic stem cells (USSCs).

Conflict of interest statement

The Authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1. Attachment and proliferation of the osteogenic USSC-18, -8 and -8/77 cell lines growing on different membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend®, BioMend® Extend™ und GORE-TEX®) for 1, 3, and 7 days. ***p<0.0005.
Figure 2
Figure 2. Viability of the osteogenic USSC-18, -8 and -8/77-cell lines growing on different membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend®, BioMend® Extend™ and GORE-TEX®) for 1, 3, and 7 days. ***p<0.0005, Fluorescence (560Ex/590Em).
Figure 3
Figure 3. Relative proliferation rates of the osteogenic USSC-18, -8 and -8/77-cell lines on different membranes (Bio-Gide®, RESODONT®, GENTAFOIL resorb®, BioMend®, BioMend® Extend™ and GORE-TEX®) between days 1 and 3, days 3 and 7 and between days 1 and 7 in comparison to the cell count on day 1 (p<0.005).
Figure 4
Figure 4. Cytotoxicity on a standard cell surface and on the membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb® and GORE-TEX®) on the osteogenic differentiated USSC-18, -8 and -8/77-cell lines after days 1, 3 and 7. ***p<0.0005, **p<0.005.
Figure 5
Figure 5. Raster electron microscope images of the studied membranes, native and colonized with USSC-18, for days 1, 3 and 7 (magnification 500×). For day 7, a representative cell has been marked in red. Cell structure, form, and size differed depending on the membrane surface. White arrow: cell, black arrow: cell extension.

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