Production of interferon gamma by peripheral blood mononuclear cells from normal subjects and from patients with rheumatoid arthritis

Clin Exp Rheumatol. Oct-Dec 1988;6(4):347-54.

Abstract

A radioimmunoassay for human interferon gamma (IFN-gamma) has been carried out using a recombinant glycosylated interferon (Hu IFN-gamma) as tracer, the N.I.H. reference preparation (Gg 23-901-530) and a polyclonal rabbit antiserum. The assay is highly specific for IFN-gamma: there is no cross-reaction either with interferons alpha and beta, Interleukins 1 and 2, tumor necrosis factor alpha and beta or with various brain peptides. The sequential saturation procedure allowed a sensitivity of 0.4 U/ml with intra and between assay coefficients of variation less than 8 and 12%, respectively. The in-vitro production of IFN-gamma by peripheral blood mononuclear cells (P.B.M.C.) was also measured. In unstimulated cultures, IFN-gamma production remained undetectable, i.e. below the 0.4 U/ml sensitivity level. After stimulation of P.B.M.C. from normal subjects with increasing amounts of PHA, both the 3H-thymidine incorporation and IFN-gamma release followed bell-shaped curves. There was no significant difference of 3H-thymidine incorporation between PHA stimulated cultures (0.2 and 2.5 ug/ml) from normal subjects (36 cases) and those with active (16 cases) or non-active (14 cases) rheumatoid arthritis. At two PHA concentrations of 0.2 and 2.5 ug/ml, mononuclear cells from patients with active disease produced significantly less IFN-gamma than those from either controls or cases with non-active disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Arthritis, Rheumatoid / immunology*
  • Cells, Cultured
  • Cricetinae
  • Humans
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / blood*
  • Iodine Radioisotopes
  • Leukocytes, Mononuclear / metabolism*
  • Lymphocyte Activation / drug effects
  • Middle Aged
  • Osteoarthritis / immunology
  • Phytohemagglutinins
  • Radioimmunoassay

Substances

  • Iodine Radioisotopes
  • Phytohemagglutinins
  • Interferon-gamma