Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

Jonathan E Wosen; Alexandra Ilstad-Minnihan; Julia Y Co; Wei Jiang; Dhriti Mukhopadhyay; Nielsen Q Fernandez-Becker; Calvin J Kuo; Manuel R Amieva; Elizabeth D Mellins

doi:10.3389/fimmu.2019.01970

Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

Front Immunol. 2019 Aug 20;10:1970. doi: 10.3389/fimmu.2019.01970. eCollection 2019.

Authors

Jonathan E Wosen¹, Alexandra Ilstad-Minnihan¹, Julia Y Co², Wei Jiang¹, Dhriti Mukhopadhyay¹, Nielsen Q Fernandez-Becker³, Calvin J Kuo⁴, Manuel R Amieva^{2

5}, Elizabeth D Mellins¹

Affiliations

¹ Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
² Division of Infectious Diseases, Department of Pediatrics, Stanford University, Stanford, CA, United States.
³ Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, CA, United States.
⁴ Division of Hematology, Department of Medicine, Stanford University, Stanford, CA, United States.
⁵ Department of Microbiology and Immunology, Stanford University, Stanford, CA, United States.

Abstract

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.

Keywords: MHC-II; antigen presentation; enteroid culture; epithelial cells; mucosal immunity.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Histocompatibility Antigens Class II*
Humans
Intestinal Mucosa / immunology*
Organoids / immunology*

Substances

Histocompatibility Antigens Class II

Abstract

Publication types

MeSH terms

Substances

Grant support