Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

Front Immunol. 2019 Aug 20;10:1970. doi: 10.3389/fimmu.2019.01970. eCollection 2019.

Abstract

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.

Keywords: MHC-II; antigen presentation; enteroid culture; epithelial cells; mucosal immunity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Histocompatibility Antigens Class II*
  • Humans
  • Intestinal Mucosa / immunology*
  • Organoids / immunology*

Substances

  • Histocompatibility Antigens Class II