X-Ray Crystallography in Structure-Function Characterization of Therapeutic Enzymes

Adv Exp Med Biol. 2019;1148:81-103. doi: 10.1007/978-981-13-7709-9_4.

Abstract

Enzymes are key biological macromolecules that support life by accelerating the conversion of target molecules to desired products in many biochemical reactions. Enzymes are characterized by high affinity, specificity and great catalytic efficiency. Owing to their unique characteristics, enzymes have attracted significant attention for use in therapeutic settings as a distinct class of drugs different from other types of medicines. Enzyme-based therapies are currently in use for the treatment of a wide range of diseases, including leukemia, metabolic disorders, inflammation and cardiovascular disease. However, several challenges, such as immunogenicity and stability, remain. X-ray crystallography has provided key structural insights into the understanding of the molecular basis of diseases and development of enzyme-based therapies. Here, the role of X-ray crystallography in the development of therapeutic enzymes is examined and several examples are provided.

Keywords: Chaperones; Enzyme deficiency; Enzyme stability; Enzyme therapy; Structure stabilizers.

Publication types

  • Review

MeSH terms

  • Crystallography, X-Ray*
  • Enzymes / chemistry*
  • Enzymes / pharmacology
  • Structure-Activity Relationship

Substances

  • Enzymes