Nephroprotective effect of Ferulago angulata flowers on N-nitrosodimethylamine-induced nephrotoxicity in rats and its phytochemical profile

J Food Biochem. 2019 Nov;43(11):e13030. doi: 10.1111/jfbc.13030. Epub 2019 Sep 3.


The present study was designed to assess the phytochemical content of Ferulago angulata (FA) and possible in vivo nephroprotective effect of FA administration on trace elements, minerals, MDA and GSH in kidney and liver tissue samples, serum vitamin (α-tocopherol, retinol, cholecalciferol, phylloquinone), TSA, and LSA in a rat model of DMN-induced nephrotoxicity. In the study, Wistar albino rats were assigned to six groups: Control (0.9% NaCl), (DMN 10 mg/kg), (FA 150 mg/kg), (DMN + FA 150 mg/kg), (FA 300 mg/kg), and (DMN + FA 300 mg/kg). Rats were intraperitoneally given DMN for the first 7 days. Renal injury caused by DMN was proved by the histopathological alterations. The FA (300 mg/kg) treatment significantly normalized Se, Cr, Ca levels in liver and Co level in kidney tissue samples. These observed positive effects are due to the phytochemical content of the plant. The flower extract of FA (300 mg/kg) can be used for the prevention of kidney damage. PRACTICAL APPLICATIONS: Ferulago angulata flowers are used in traditional medicine for treat kidney and liver digestive system diseases. This species is endemic taxa of the family Apiaceae, which has been used both as food and therapeutics because of their phytochemical composition. In this study, the phenolic characterization of FA flower was used to a new RP-HPLC method, as well as the biological activity of FA flower and possible in vivo nephroprotective effect of FA flowers on trace elements, minerals, MDA and GSH in kidney and liver tissue samples and vitamins, TSA, and LSA in serum samples a rat model of DMN-induced nephrotoxicity. It was found that high level of phenolic compounds (chlorogenic acid, vanillic acid, 2-hydroxycinnamic acid) present in the flower extract of F. angulata has positive effects and antioxidant properties. Due to its phenolic content, FA flower extract could protect for kidney damage and can be used as antioxidants in the food additive and pharmaceutical industry.

Keywords: Ferulago angulata; DMN; kidney; phenolics; trace element; vitamin.

MeSH terms

  • Animals
  • Apiaceae / chemistry*
  • Dimethylnitrosamine / toxicity*
  • Flowers / chemistry
  • Glutathione / metabolism
  • Humans
  • Kidney / drug effects
  • Kidney Diseases / etiology
  • Kidney Diseases / metabolism
  • Kidney Diseases / prevention & control*
  • Male
  • Oxidative Stress / drug effects
  • Phenols / administration & dosage
  • Phenols / chemistry
  • Phytochemicals / administration & dosage*
  • Phytochemicals / chemistry
  • Plant Extracts / administration & dosage*
  • Plant Extracts / chemistry
  • Protective Agents / administration & dosage*
  • Protective Agents / chemistry
  • Rats
  • Rats, Wistar


  • Phenols
  • Phytochemicals
  • Plant Extracts
  • Protective Agents
  • Glutathione
  • Dimethylnitrosamine