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. 2019 Aug 21;5(8):e02275.
doi: 10.1016/j.heliyon.2019.e02275. eCollection 2019 Aug.

Antidiabetic, Antioxidant and Antimicrobial Activities of Extracts of Tephrosia bracteolata Leaves

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Free PMC article

Antidiabetic, Antioxidant and Antimicrobial Activities of Extracts of Tephrosia bracteolata Leaves

Godshelp Osas Egharevba et al. Heliyon. .
Free PMC article

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Abstract

Aims: Plant extracts have long been used for the ethnomedical treatment of diabetes, microbial infections and as a source of antioxidant. This study was aimed at investigating the antidiabetic, antioxidant, and antimicrobial activities of the n-hexane and ethyl acetate extract of Tephrosia bracteolata leaves (TBL) as associated with the ethnobotanical knowledge of the local people of Nigeria.

Main methods: The phytochemical composition of the n-hexane and ethyl acetate extract of the leaves of T. bracteolata were determined following standard procedures in literature, and it's in vitro inhibitory activities against α-glucosidase enzyme. 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS.+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH+) antioxidant activities were also examined. Well diffusion method was employed in evaluating the antimicrobial property of the extracts.

Key findings: The ethyl acetate extract of T. bracteolata leaves had the greatest inhibitory effect on α-glucosidase, followed by the n-hexane with IC50 43.95 μg/ml and IC50 ˃50 μg/ml respectively. The ethyl acetate also exhibited significant DPPH+ and ABTS.+ antioxidant activity with IC50 of 24.96 μg/ml and 6.48 μg/ml as compared to Ascorbic acid and Trolox (12.24 μg/ml and 5.91 μg/ml) respectively. The zones of inhibition of the ethyl acetate extract of T. bracteolata leaves ranges from 10 - 25 mm at a concentration of 6.25-200 mg/ml, and it showed a greater antibacterial activity than the n-hexane extract, having a zone of inhibition from 10 - 20 mm at concentration of 12.5-200 mg/ml when compared to the standard Gentamycin. Similarly, the ethyl acetate extract of T. bracteolata showed a better anti fungi activity at concentration range 12.5-200 mg/ml than the n-hexane extract at concentration range of 25-200 mg/ml with reference to Tioconazole. These results indicated for the first time that the ethyl acetate extract of T. bracteolata leaves extracts exerted potent inhibitory effects against α-glucosidase, actively scavenge DPPH+ and ABTS.+ free radicals and successfully inhibits the proliferation of Gram positive and Gram negative microorganism.

Significance: TBL is an important source of antidiabetic, antimicrobial and antioxidant agent.

Keywords: Agriculture; Alpha glucosidase; Antidiabetic; Antimicrobial; Antioxidants; Microbiology; Phytochemical composition; Plant biology; Tephrosia bracteolata.

Figures

Fig. 1
Fig. 1
α - Glucosidase % Inhibition of TbHL and TbEAL.
Fig. 2
Fig. 2
IC50 Value of TbHL and TbEAL Extracts on α – Glucosidase Inhibitory Assay.
Fig. 3
Fig. 3
% inhibition of DPPH and ABTS + assay respectively.
Fig. 4
Fig. 4
IC50 value of DPPH anad ABTS + Assay.

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