A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Methods Mol Biol. 2020;2058:127-132. doi: 10.1007/978-1-4939-9794-7_8.

Abstract

Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).

Keywords: Antigen-presenting cells; Dendritic cells; Flow cytometry; Immunogenic cell death; Oncolytic viruses.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • Cell Death / immunology
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Genetic Therapy / methods
  • Genetic Vectors / genetics*
  • Humans
  • Immunomodulation*
  • Immunophenotyping
  • Monocytes / immunology
  • Monocytes / metabolism
  • Neoplasms / immunology*
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Neoplasms / therapy
  • Oncolytic Virotherapy
  • Oncolytic Viruses / genetics*
  • Oncolytic Viruses / immunology