Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation

Methods Mol Biol. 2020;2058:133-154. doi: 10.1007/978-1-4939-9794-7_9.


Newcastle disease virus (NDV) is an avian paramyxovirus that has been extensively studied as an oncolytic agent, in addition to being an economically important pathogen in the poultry industry. The establishment of a reverse genetics system for this virus has enabled the development of genetically modified recombinant NDV viruses with improved oncolytic and immunotherapeutic properties. In this chapter, we describe the materials and methods involved in the in vitro cloning and rescue of NDV expressing murine 4-1BBL as well as the in vivo evaluation of NDV expressing 4-1BBL in a B16-F10 murine melanoma model.

Keywords: Immunotherapy; Newcastle disease virus; Oncolytic vector; Reverse genetics; Virus rescue.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Gene Expression
  • Genetic Engineering*
  • Genetic Therapy / methods
  • Genetic Vectors / genetics*
  • Humans
  • Immunomodulation* / genetics
  • Melanoma, Experimental
  • Mice
  • Neoplasms / immunology
  • Neoplasms / metabolism
  • Neoplasms / therapy
  • Newcastle disease virus / genetics*
  • Newcastle disease virus / immunology
  • Oncolytic Virotherapy / methods
  • Oncolytic Viruses / genetics*
  • Oncolytic Viruses / immunology
  • Transgenes